The ProtekDuo as Double Lumen Return Cannula in V?VP ECMO Configuration: A First?In?Man Method Description

We present a case of acute respiratory distress syndrome (ARDS) secondary to COVID?19 who required venovenous extracorporeal membrane oxygenation (V?V ECMO). Initially, a right ventricular assist device (RVAD), the ProtekDuo with an oxygenator, was placed in an outside heart center and the patient was transferred to us for ECMO management. Due to severe hypoxia, the configuration was later modified, and a 25 Fr femoral drainage cannula was inserted for venous drainage only. The arterial return tubing was spliced and using a Y?connector, arterialized blood was returned through both limbs of the ProtekDuo resulting in a significantly increased oxygenation and flow.

Application of new methods on the study of ancient human remains in China in recent years / 解剖学报

In the past decade, in addition to using traditional naked-eye morphological analysis, two-dimensional measurement and data comparison, more and more scholars placed great emphasis on the application of new technologies available and supported the study of ancient human remains from a multi-disciplinary perspective. Through outlining recent advances in the application of new techniques on research of ancient human remains, this paper briefly summarized the characteristics of present research and gave an outlook on future research of ancient human remains in China.

New methods and technologies in the field of perforator flap studies / 中华整形外科杂志

A large number of innovative and new technologies and method in the field of perforator flap were proposed with the hard work of many experts and surgeons. This paper briefly reviewed the following items including the discovery of the perforator flap and its enlightenment to us, three-dimensional visualized anatomy, angiosome and perforasome theories, pre-operative perforator detection techniques, techniques for evaluation of the flap vascular perfusion, the concept of freestyle perforator flap, chimeric flap, flow-through flap technique, new designs of pedicle perforator flap, particularly the propeller and keystone flaps, supramicrosurgery, superthin flap, microdissected thin perforator flap, and microvascular breast reconstruction and lymph node transfer for postmastectomy lymphedema patients. These new technologies and method have greatly improved our understanding of flap surgery and promote the development of the reconstructive surgery. The traditional research focused on investigating the anatomic features of a single flap and strategies for the reconstruction of different defects and organs. Nowadays, the treatment scope of reconstructive surgery is more extensive and has developed to repair various refractory wounds following trauma and oncological resection, nerve injuries, and to reconstruct defect and organ by using the vascularized composite allotransplantation. Researches on the upper limb lymphedema after breast cancer surgery have been highly valued recently. It can be expected that the direction of reconstructive surgery would be transformed from the improvement of theraputic modalities to concentrating on the treatment of diseases, and this undoubtedly conforms to the essence of medicine. The research of perforator flaps will be directed to more precise, minimally invasive, and individualized according to the requirements of evidence-based medicine.

Placa infrapectínea para reparación con mínima invasión de fracturas de lámina cuadrilátera acetabular: descripción de un nuevo implante y acceso paramedial / Infrapectineal Plate for repair with minimal invasion of quadrilateral blade acetabular fractures: description new implant and paramedial access

Resumen: Introducción: Las fracturas de la lámina cuadrilátera del acetábulo son las más difíciles de reducir y fijar. Se han desarrollado diferentes técnicas para la osteosíntesis de la lámina cuadrilátera. El objetivo de este trabajo fue crear implantes y un nuevo acceso quirúrgico para simplificar y mejorar la osteosíntesis de fracturas del acetábulo. Material y métodos: Un total de 83 pacientes fueron estudiados mediante la tomografía axial computarizada de ambos acetábulos, siendo medidos a nivel de columna posterior alta y baja con el fin de determinar longitud y diámetro de los implantes, a través del análisis de normalidad de variables, dónde p es > 0.05, usando la prueba Kolmogorov-Smirnov (Lilliefors). Las características anatómicas del nuevo acceso quirúrgico también se describen. La incisión se practicó en espécimen cadavérico para determinar la seguridad de todo el acceso. Resultados: El par de tornillos macho-hembra midió 20 x 6 x 8 mm (longitud, diámetro interno y de la cabeza), mientras que las placas fueron de 10 mm de ancho y 3 mm de espesor, con longitud correspondiente al número de orificios. Se desarrollaron instrumentos apropiados para su aplicación. Discusión: Este método puede facilitar la osteosíntesis del acetábulo. Se requieren estudios cadavéricos y clínicos para corroborarlo. Puede ser que se mejoren los resultados de osteosíntesis del acetábulo, con menor riesgo.

Abstract: Introduction: Quadrilateral plate fractures are the most difficult to reduce and fix. Different techniques have been developed for quadrilateral plate osteosynthesis. The objective of this work was to create an implant and a novel approach to simplify and improve acetabular fracture osteosynthesis. Material and methods: A total of 83 patients were studied. Pelvic CT scan images of both acetabula were measured at the proximal and distal posterior column. Implant length, diameters and morphological characteristics were determined. The anatomical features of a novel surgical approach are described. The paramedian approach was performed on a cadaveric specimen to determine its anatomical safety. Results: The screws measured 20 × 6 × 8 mm (length × core diameter x head diameter), with internal threads of 4.5 mm. The Kolmogorov-Smirnov (Lilliefors) test was used, where p had to be > 0.05. Plates were previously determined to be 10 mm wide and 3 mm thick, of variable length. Instruments were developed to surmount difficulties. Discussion: This new procedure and implant could make the repair of acetabular fractures easier and offers several advantages. Clinical trials are needed to assess the benefits of this proposal. The newly described method can allow acetabular fracture osteosynthesis to be performed safely, avoid iatrogenic injury to anatomical structures and achieve better results.

Single-cell analysis method for twitching motility of Pseudomonas aeruginosa / 生物工程学报

Twitching motility is very important for Pseudomonas aeruginosa in the adaptation of surface environment and in the 3-D structure formation of mature biofilm. To quantitatively characterize twitching motility in situ, we developed a method by combining high-throughput data acquisition, automatic image processing and database establishment. This method is based on single cell analysis and big data visualization. A periodic relaxation of 0.9 second was resolved during slingshot motility analysis. Twitching motilities of bacteria under addition of two quorum sensing signaling molecules were studied, cells moved faster after signal addition. This method may help understand the molecular mechanism and regulatory circuits of twitching motility.

A new family home approach to controlling iron deficiency anemia in all ages in less-developed and developing countries using iron-fortified water

Nutritional iron deficiency anemia is considered the main public health problem of poor less-developed and developing countries. The World Health Organization has estimated that 1.5-2.0 billion persons are anemic. It has been said that close to 1 million deaths are linked to iron deficiency anemia. The groups most vulnerable to this form of anemia are said to be small children and women of reproductive age. Our goal is to show that iron fortification in the water, will control anemia. The method used was a literature review. Methods have been identified to control anemia, but the fortification of water is a more efficient, low cost, reaches the entire population, prevents and treats deficiency anemia. We concluded that the iron fortification of drinking water at home is a simple, effective, and low-priced approach for the prevention of iron deficiency anemia prevalent in poor and developing countries(AU)

A anemia por deficiência nutricional de ferro é considerada o principal problema de saúde pública dos países menos-desenvolvidos e em desenvolvimento. A Organização Mundial de Saúde estimou que 1,5-2,0 milhões de pessoas são anêmicas. Aproximadamente 1 milhão de mortes estão ligadas a anemia por deficiência de ferro. Os grupos mais vulneráveis a anemia são crianças e mulheres em idade reprodutiva. Nosso objetivo é mostrar que fortificação de ferro na água, irá controlar anemia. O método utilizado foi revisão da literatura. Diversos métodos têm sido identificados para controle da anemia, mas a fortificação da água é uma forma mais eficiente e de baixo custo, atingindo toda a população, previnindo e tratando a anemia por deficiência. Concluímos que a fortificação de ferro na água potável em casa é uma abordagem simples, eficaz e de baixo custo para a prevenção da anemia ferropriva prevalente nos países pobres e em desenvolvimento(AU)

A new method of mouse tracheal instillation with endoscope of cold light and gel loading tips / 中国药理学通报

Aim To develop a new method for easy endotrache-al intubation and instillation in mice. Methods Relying on the endoscope of cold light and gel loading tips, a new method was set up for intubated operations. Under high magnification, oper-ations of endotracheal intubation and instillation were completed in mice. Twenty male CB17-SCID mice ( between 24 and 28 g) were repeatedly intubated and instilled with 80 μL suspensions of air polluted fine particulate matters in a week. Results The twenty mice were sacrificed after 24h of the last instillation. The dispersions of the air polluted fine particulate matters were found at the bottoms of tracheas and in both the left and right lungs in all mice. Conclusion Using the endoscope of cold light and gel loading tips, a new method for tracheal instillation is set up in mice, which is rapid, simple and has good repeatability. It could be widely applied for the endotracheal intubation and in-stillation in mice.

Comparison of Several Methods and Their Progresses on Molecular Authentication of Lonicera japonica Thunb / 世界科学技术-中医药现代化

Authentications of Chinese herbal medicine have a critical effect in Chinese clinical medicine. DNA molecular marker, as an important component for true or false authentication, is more and more widely used in iden-tification of Chinese medicinal materials. At the same time, many new methods for authentication of Chinese medici-nal materials are continuously emerging. But the systematically comparative analysis of these new methods is lack. The present study taking Lonicera japonica as an example, systematically compared principles, characteristics, ex-periment methods, detection time and the application scope of express sequence tag-simple sequence repeat (EST-SSR), polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), allele-specific PCR (AS-PCR), DNA barcoding and loop-mediated isothermal amplification (LAMP), and put forward corresponding improve-ment opinions. This study can help to screen appropriate approach for rapid authentication of L. japonica and offer demonstrating to other Chinese herbal medicines.

Development and Validation of New RP-UPLC Method for the Quantitative Analysis of Mycophenolate in Tablet Dosage Form.

Aim: To develop and validate a novel and rapid reverse phase ultra-performance liquid chromatographic (RP-UPLC) method for the estimation of mycophenolate in tablet formulations. Place and Duration of Study: Department of pharmaceutical analysis, S. R. College of Pharmacy ananthasagar, between February 2012 and June 2012. Methodology: Chromatographic separation was achieved on a Symmetry C18 (4.6x100mm, 3.5μm, Make: XBridge) column using an isocratic method with mobile phase composed of Potassium di-hydrogen phosphate: Acetonitrile in the ratio 35:65 v/v). The flow rate was 0.2 ml/min, temperature of the column was maintained at ambient and detection was made at 228 nm. The run time was as short as 1.2 min. The developed method was validated according to the International Conference on Harmonization (ICH) guidelines with respect to linearity, accuracy, precision, specificity and robustness. Results: The developed method was linear for mycophenolate from 10-50μg/ml and the linear regression obtained was > 0.999. Precision, evaluated by intra- and inter-day assays had relative standard deviation (R.S.D) values within 1.5%. Recovery data were in the range 99.1-102.0% with R.S.D. values < 1.5%. Conclusion: The method is precise, accurate, linear, robust and fast. The short retention time of 0.613 min allows the analysis of a large number of samples in a short period of time and, therefore, should be cost-effective for routine analysis in the pharmaceutical industry.

Some methods of measuring patients adherence to medication / 中国药学杂志

OBJECTIVE: To introduce some methods to measure patients' adherence to medication, including the traditional and modern methods, so that clinical pharmacists can provide appropriate interventions to improve patients' adherence. METHODS: Methods to measure patients' adherence were reviewed. RESULTS AND CONCLUSION: The methods to measure patients' adherence have developed a lot. Measuring patients' adherence is especially important for chronic disease management. More researches are needed to develop more accurate methods to measure patients' adherence.

Schistosoma mansoni: a method for inducing resistance to praziquantel using infected Biomphalaria glabrata snails

To elucidate the mechanisms of antischistosoma resistance, drug-resistant Schistosoma mansoni laboratory isolates are essential. We developed a new method for inducing resistance to praziquantel (PZQ) using successive drug treatments of Biomphalaria glabrata snails infected with S. mansoni. Infected B. glabrata were treated three times with 100 mg/kg PZQ for five consecutive days with a one-week interval between them. After the treatment, the cercariae (LE-PZQ) produced from these snails and the LE strains (susceptible) were used to infect mice. Forty-five days after infection, mice were treated with 200, 400 or 800 mg/kg PZQ. Thirty days post-treatment, we observed that the mean number of worms recovered by perfusion was significantly higher in the group of mice infected with the LE-PZQ isolate treated with 200 and 400 mg/kg in comparison to the LE strain with the same treatment. Moreover, there was a significant difference between the ED50 (effective dose required to kill 50 percent of the worms) of the LE-PZQ isolate (362 mg/kg) and the LE strain (68 mg/kg). In the in vitro assays, the worms of the LE-PZQ isolate were also less susceptible to PZQ. Thus, the use of infected snails as an experimental model for development of resistance to S. mansoni is effective, fast, simple and cheap.

A new method in the diagnosis of lymphnode metastasis in patients with colorectal cancer / 国际外科学杂志

Forecasting the colorectal cancer prognosis mainly based on the accurate pathological stages,the primary carcinoma infiltrated depth,the cell differentiation degree and the capillaries infiltrated in histology result,and the outcome can be obtained latter 3-7 days after the opemtion.But in traditional lymph node inspection method,identifying the quantity and the anatomy locality of metastasized lymph node normally costs long time,sometimes cannot obtain the result.M addition,to identify the stage of the cancer is a key point to determine the assistant chemotherapy is useful or not,for both doctor and patient,because the excessive treatment and the insufficient treatment are both harmful to the patient.Now overseas has an abvoad study showes that regional lymph node metastasis have something to do with the concentration of(Carcinembryonic,CEA)in the lymph node.The CEA concentration can be obtained simply and directly.So,testing the CEA concentration in lymph node may become a new method in the diagnosis of lymph node metastasis for patients with colorectal cancer.

Study of New Therapy Method to Decrease the Relapse of Genital Herpes with Laser / 医学研究杂志

Objective To explore new therapy method to reduce relapse of genital herpes(GH) with laser.Methods 550 cases GH treated with Laser were randomly derided into 11 different groups(each group 50 cases: group A(A.T.GH.Q),B(B.TGH.Q),C(C.T.GH.Q),D(D.TGH.Q),E(E.T.GH.Q),F(F.TGH.Q),G(G.T.GH.Q),H(H.T.GH.Q),I(I.T.GH.Q),J(J.T.GH.Q) and K group(K.T.GH.Q).All of cases started with 2~8 years of follow-up after clinical short-term being cured.Results Cure rate were: A 24%,B 22%,C 32%,D 30%,E 36%,F 448%,G 42%,H 48%,I 60%,J 58%,K 18%.Conclusion GH treated by Laser auompanied by drug has good and ideal long-term effectiveness,worthwhile for further study and popularized clinical application.

Influence factor analysis of application of the new gray correlation analysis method to hospitalization costs / 中华医学教育探索杂志

Objective By investigating the influencing factors and the tendency of devel-opment of 6-year hospitalization costs in our hospital,our hospital explores an appropriate way of analysis on the influencing factors of medical costs,in order to offer some quantifiable evidence of evaluating and meaningful references to controlling medical costs. Methods to use the new method of gray correlation analysis to make the quantitative analysis of the composing of the hospital fees from 2002 to 2007.Results the new method of gray correlation analysis is able to overcome the influence of non-dimensional treatment which affects the role of factors and simplifies the calculation steps. Conclusion It is a statistical analysis method whose result is reasonable and reliable,whose application is flexible and simple. It can also offer a quantifiable index of the tendency of the system of development and changes. It is suitable for measuring the interrelated degree among the factors and the dynamic process of analysis.

Function of Lead Block Validating Instrument in Radiotherapy / 医疗卫生装备

Objective To investigate and carry out the function of the lead block validating instrument in the radiotherapy. Methods Based on clinical practice and the principle comparative steady STD and SAD of linear accelerator, the function of lead block validating instrument was developed in reason. Results The working quality and efficiency are greatly improved. Conclusion Not only the criterion extent of founded lead block and the proof of every kinds spume blocks in three-dimensional conformal radiotherapy and integer block radiotherapy and electron radiotherapy, but also the shadow tray centers and center reticle can be validated by the lead block validating instrument.

New Operative Method for Transsphincteric Fistula

PURPOSE: The complications after surgery for trans sphincteric anal fistula are anal incontinence due to cutting of the external anal sphincter muscle and recurrent anal fistula. Several methods have been developed to alleviate this condition, but they still have many complications. We performed surgery for transsphincteric anal fistula by a new method with excellent results and therefore report this new method as another treatment modality for transsphincteric anal fistula. METHODS: We performed surgery for transsphincteric anal fistula on 12 patients at Hanyang University Kuri Hospital between March 1999 and December 2001. RESULTS: There were no recurrences in any of the 12 patients, all of whom kept continence after surgery. Our operative method involve coring out and fistulectomy, laying open, closure of the defected external sphincter muscle and partial closure of the skin of the external opening. CONCLUSION: Our simple and modified method for transsphincteric anal fistula showed excellent results, especially in terms of the rate of recurrence and fecal incontinence. We therefore recommended this easy and simple method for surgery for transsphincteric anal fistula.

Partial Cryoablation on Retionpathy of Prematurity

Although the reports of the use of crotherapy in the control of the proliferative phase of retinopathy of prematurity varied in their conclusions regarding the merits and limitations of this therapy, cryotherapy has been consedered to be an effective treatment for retinopathy stage 3. The accepted method of therapy includes ablation of the whole avascular retina from the ridge to the ora serrata. This is achieved by 2 to 3 rows of cryoapplications with use of cryotherapy probe for ROP. Nissenkorn et al presented their new method of cryotherapy in which only one row of cryoapplications was applied in the avascular retina anterior to the fibrovascular ridge. So, we performed cryotherapy using Nessenkorn's new method on 7 babies and the anatomical results are reviewed. Complete regression of active retinopathy was achieved in all of the eyes treated.